Bill of Materials?

Gerrit

I may have missed this somewhere, but do we have a list of components needed to build the setup described in @Martin 's latest video?

For example, where can I download @SandrineH 's Sodastream holder? And I'd like to make sure I get all the same other bits and pieces as well so that we're building the same setups.

Martin

OK, here's a work in progress, I'll edit this until I think I've got it right at which point I'll delete this paragraph and add a second post to notify anyone watching this thread.

Bill of Materials

1x Pioreactor + Raspberry Pi ¹
1x Dovetail platform for Raspberry Pi ²
CO2 sparging
1x Sodastream cylinder
1x Dovetail Sodastream cylinder holder - @SandrineH would you be happy to publish your design?
1x W21.8-14 to TR21-4 adaptor ³
1x CO2 Regulator and Solenoid ⁴
~ 20cm 4mm PU tubing
2x Check valves
1x 3/16" barb male Luer lock
1/16" barb Luer Locks - Male & Female
~ 20cm 1/16" ID Silicone tubing
1x 2.1mm x 5.5mm Male Barrel Plug - to connect Raspberry Pi to solenoid with:
4x Female Dupont Cables (also needed for electrolysis)
Electrolysis
4 port + 2 M4 threads vial cap - @RobJ would you be willing to publish your design?
2x Stainless Steel 70mm M4 bolts
2x Stainless Steel M4 Locking Nuts - I bought mine in a set
2x Ring Crimp Terminals ⁵
1 strip cut from a [Platinized Titanium Anode]
Reagents
See this post

Future requirements (not used in the above video)

To allow continuous operation (chemostat/turbidostat) once the culture is established:

To monitor colour (absolutely optional, possibly not even useful, watch this space)

1x Adafruit Spectrometer
1x Stemma QT cable

Other extras

Spare 20ml glass vials

Tools

1-5ml Pipettor & tips (I also got a 10-100 ul pipettor and tips but didn't use in this experiment)
Crimping Pliers - both for crimping and bolt cutting
150mm Pliers Wrench
Swiss Army Knife Pliers
Nitrile Gloves
PH0 Screwdriver
Spatula
Weighing Boat
Jewellers Scales
1L volumetric flask
1L Reagent Bottle
1L Amber Reagent Bottle
1L Wash Bottle

-

¹ I used a Raspberry Pi 4 but only because my studio setup requires ethernet rather than WiFi
² actually mine was modified by @CamDavidsonPilon to fit the Ethernet cable (Cam, is that mod available online? ***Answer yes: here)
³ could be improved, I got this as it is also a 90° bend, but mine is uncomfortably stiff to adjust
⁴ would we be better off with a pinch valve? Can we produce an open source pinch valve based on a widely available solenoid? If so could a 5v solenoid suffice? That would mean we don't need a second power adapter
⁵ I don't think I'd recommend the crimp terminal set I bought

CamDavidsonPilon

Martin Yes! We just added an ethernet cut out version here: https://www.printables.com/model/679663-pioreactor-platform-with-dovetails-for-b-models/files

Martin

CamDavidsonPilon Thanks Cam

SandrineH

@RobJ and I are both happy with sharing the designs publicly

Martin

SandrineH Thanks Sandrine, would you like me to open an AMYBO account on Printables and upload them there, or would you rather upload them to your own accounts?

Gerrit

@Martin @SandrineH Any consensus on who will publish the 3D printed parts?

Martin

Gerrit @Rob said he had spoken with @SandrineH and I could. But he also said he'd comment on his licensing thread, so I thought it sensible to wait for that.